First evidence of high-molecular-weight bacteriocin (tailocin) produced by Antarctic Pseudomonas spp.

Kateřina Snopková, Ivo Sedláček, David Šmajs

10.5817/CPR2018-2-14

Cold-adapted soil ecosystems represent dynamic communities varying in a structure, microbial abundance and metabolic activity. To antagonize competitors, soil bacteria produce a variety of inhibitory agents. We tested production of antimicrobials in Pseudomonas spp. isolated in James Ross Island, Antarctica, and performed transmission electron microscopic analyses of selected high-molecular-weight bacteriocin particles. The dimensions of R-tailocins produced by Pseudomonas sp. P2422 were 168 ± 2.0 nm (length) and 16 ± 0.8 nm (width) thus representing one of the largest tailocins secreted by Pseudomonas spp. To our knowledge, this is the first evidence of tailocin production by bacteria originated from polar regions.

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