Medium 101: GC AGAR BASE

Commercial: GC Agar Base Oxoid CM0367, Growth 
supplement VITOX Oxoid SR0090A and Soluble 
Haemoglobin Powder Oxoid LP0053 

    GC agar base                  18 g
    Distilled water              240 ml
    Sterilize at 121°C for 15 min.

Cool to 50°C and add aseptically:

    Haemoglobin* (2%)            250 ml
    Growth Supplement VITOX**     10 ml 

   *Haemoglobin solution:
    Soluble Haemoglobin Powder     5 g
    Distilled water              250 ml
    Sterilize at 121°C for 15 min and cool to 
50°C.

  **Growth Supplement VITOX:
    Dissolve the content of 1 vial of VITOX in 10 
ml VITOX Rehydratation Fluid.