Medium 118: PYA MEDIUM

I.  Pepton                          8 g
    Yeast extract                   3 g
    KH2PO4                          1 g
    Agar                           20 g
    Distilled water               800 ml	

    Sterilize at 121°C for 15 min.

II. Na2CO3                         10 g
    Distilled water               200 ml

    Sterilize through Seitz filter.
    
    Sterilize separately solution II and add 
after
    cooling to solution I.
   
    Check final pH to be 10.0 - 10.2