Proteose Peptone (Difco No.3) 0.5 g Casamino acids 0.5 g Glucose 0.5 g Tween 80 1 ml Sodium pyruvate 0.3 g K2HPO4 0.3 g MgSO4.7H2O 0.05 g Agar 20 g Distilled water 1000 ml pH 7.2 (adjust with crystalline K2HPO4 or KH2PO4 before adding agar). Add agar, heat medium to boiling to dissolve agar.