Medium 131: CDM MEDIUM

	I. Solution
	MgSO4.7H20                     2 g
	NH4Cl                          1 g
	Na2SO4                      0.99 g
	K2HPO4                      0.01 g
	CaCl2.2H20                 0.067 g
	Na lactate                  0.05 g
	Deionized water              100 ml
	Sterilization: at 121°C for 15 min.

	II. Solution:
	FeSO4.7H20                  0.13 g
	Deionized water              100 ml
	Sterilization: Millipore filter

	III. Solution:
	NaHCO3                      7.98 g
	Deionized water              100 ml
	Sterilization: Millipore filter

Add 20 g the agar and 900 ml distilled water to 
solution I and sterilizite at 120 C for 15 min., 
final pH 7.2. Add aseptically 2.5 ml solution II. 
and 10 ml solution III. to the warm solution I. 
with the agar and distribute the medium into 
sterile tubes or Petri dishes.