Medium 168: ATCC MEDIUM 112 (VAN NEL'S YEAST AGAR)

    K2HPO4                1 g
    MgSO4               0.5 g
    Yeast Extract        10 g
    Agar                 20 g (or 10 g)
    Distilled water    1000 ml 

    Adjust pH to 7.0 - 7.2
    Sterilized at 121°C for 20 min