Medium 176: R2A 7.5% SALT MEDIUM

Yeast extract          0.05 g
Protease Peptone No.3  0.05 g
Casamino Acids         0.05 g
Dextrose or glucose    0.05 g
Soluble starch         0.05 g
Sodium pyruvate        0.03 g
Dipotassium phosphate  0.03 g
Magnesium sulfate     0.005 g  
Agar                     20 g
Distilled water     csp 75 ml
SW30*                   25 ml

Adjust pH to 7.5

Sea Water Solution 30% (SW30)
NaCl                    234 g
MgCl2 . 6H2O             39 g
MgSO4 . 7H2O             61 g
CaCl2                     1 g
KCl                       6 g
NaHCO3                  0.2 g 
NaBr                    0.7 g
Distilled water       1000 ml