Medium 65: MINERAL MEDIUM FOR CHEMOLITHOTROPHIC GROWTH (H-3)

    KH2PO4                       2.3 g 
    Na2HPO4.2H2O                 2.9 g 
    NH4Cl                          1 g 
    MgSO4.7H2O                   0.5 g 
    NaHCO3                       0.5 g 
    CaCl2.2H2O                  0.01 g 
    Ferric ammonium citrate*    0.05 g 
    Trace element solution**       5 ml
    Agar (if needed)              20 g 
    Distilled water              980 ml
    pH 6.8 
 
*Sterilize ferric ammonium citrate (0.05 g in 20 
ml H2O) separately and then add to the medium.

    **Trace element solution:
    ZnSO4.7H2O                   0.1 g 
    MnCl2.4H2O                  0.03 g
    H3BO3                        0.3 g
    CoCl2.6H2O                   0.2 g
    CuCl2.2H2O                  0.01 g
    NiCl2.6H2O                  0.02 g 
    Na2MoO4.2H2O                0.03 g
    Distilled water             1000 ml

For chemolithotrophic growth incubate the culture 
under an atmosphere of 2% (v/v) O2, 10% CO2, 60% 
H2 and 28% N2.