I. Yeast extract 10 g
Casamino acids 7.5 g
Sodium citrate 3 g
KCl 2 g
MgSO4.7H2O 1 g
FeSO4.7H2O 0.05 g
MnSO4.4H2O 0.25 mg
NaCl 200 g
Agar 30 g
Distilled water 900 ml
Dissolve agar by heating before adding sodium
chloride.
II. Na2CO3 5 g
Distilled water 100 ml
Sterilize separately solution II. and add after
cooling to I. Check final pH to be 8.5 - 9.5.