Medium 80: ACIDITHIOBACILLUS FERROOXIDANS MEDIUM

    I. (NH4)2SO4                   2 g 
    KCl                          0.1 g 
    K2HPO4                       0.5 g 
    MgSO4.7H2O                   0.5 g 
    Ca(NO3)2                    0.01 g 
    Distilled water              700 ml 
    pH 1.8 - 2.0
 
    II. FeSO4.7H2O                40 g 
    1 N H2SO4                      2 ml 
    Distilled water              300 ml 
    pH 1.8 - 2.0 
 
Adjust pH with dilute H2SO4 or KOH. Sterilize I. 
and II. separately, mix together and distribute 
the medium into sterile Erlenmeyer flasks.