Doktorské studium

Studijní obor Fyziologie živočichů

Název práce: Multiparametrická analýza jednotlivých rakovinných buněk a jejich mikroprostředí u solidních nádorů

Školitel: Mgr. Karel Souček, Ph.D.

Předběžné zadání:
Complex immunophenotyping and identification of heterogeneity in cancer-associated stroma. We aim to identify novel markers to distinguish between normal stroma and reactive tumor stroma using BD Lyoplate Screening Panels in multicolor setup established for BD FACSVerse. Subsequently we plan to apply this knowledge to breast cancer samples and sort distinct stromal subpopulation, marked with specific antibodies against surface antigens with BD FACSAria Sorp II and analyze their transcriptome to reveal the crosstalk between cancer cells and these highly complex components of tumor microenvironment. As tumors are known as wounds that never heal, tissue components responding to injury, such as stroma and endothelium, play crucial role in progression, growth and spread of cancer. To date, only very little is known about attributes or phenotype of these heterogeneous cell populations and their contribution to cancer dissemination.


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