Doktorské studium

Studijní obor Genomika a proteomika

Název práce: Analýza funkce chromatinových komplexů SMC5/6

Školitel: doc. Mgr. Jan Paleček, Dr. rer. nat.

Oficiální zadání:
Chromosomes reside in the cell nucleus. However, the length of human DNA molecules is 10000 times bigger than the space of the nucleus. To help billions of bases fit into the small nucleus, histone and other chromatin-associated proteins come and fold DNA into condensed fibers. The SMC (Structure maintenance of chromosome) complexes are the key components of higher-order chromatin fibers and play important roles in genome stability. They are conserved from bacteria to humans. Three SMC complexes are present in most eukaryotic cells: cohesin (SMC1/3), condensin (SMC2/4) and SMC5/6 complex. Cohesin can make internal loops or embrace two sister chromatids (feature essential for proper chromosome segregation). Condensin interconnects loops to condense chromatin during mitosis. The SMC5/6 complex is involved in the homologous recombination-based DNA repair, in replication fork stability and processing, and in cohesin regulation. We study assembly and functions of SMC5/6 complexes. We use combination of genetic (fission yeast model), biochemical (mostly yeast two-hybrid system and other binding assays) and bioinformatics methods to get deep insights into their features (particularly protein-protein and protein-DNA interactions).
Literatura:
PALEČEK, Jan a Stephan GRUBER. Kite Proteins: a Superfamily of SMC/Kleisin Partners Conserved Across Bacteria, Archaea, and Eukaryotes. Structure, CAMBRIDGE: CELL PRESS, 2015, roč. 23, č. 12, s. 2183-2190. ISSN 0969-2126. doi:10.1016/j.str.2015.10.004.


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