Title: |
Microbial diversity of internal environment of Johann Gregor Mendel Station, Antarctica |
Authors Name: |
Alena Ševčíková, Jana Bednářová, Sonia Bartáková, Pavel Ševčík |
Journal: | Czech Polar Reports |
Volume: | 1 |
No.: | 1 |
Page Range: | 34-41 |
No. of Pages: | 8 |
Year: | 2011 |
DOI: |
10.5817/CPR2011-1-3 |
Publishers: | muniPress Masaryk University Brno |
ISSN: | 1805-0689 (Print), 1805-0697 (On-line) |
Language: | English |
Subject: | |
Abstract: |
During January and February
2011 two sets of microbiological samples were collected inside the main
building of Johann Gregor Mendel station located at the James Ross
Island near the coast of Antarctica. The aim was to examine the changes
of microbial profile of the antarctic station environment. The first set
of samples was collected from the station environment before the staff
entry, i.e. after 10 months of quiescent state when nobody was
present at the station. The second set of samples was collected from the
same places before the Antarctic expedition staff left the station after
45 days of the stay. The cultivation of samples was focused on
mesophilic bacteria. Twenty-three strains were obtained from the Set No.
1 and 27 strains were obtained from the Set No. 2. However, 8 strains
from each set were not reliably identified by mass spectrometry.
Altogether 13 strains of Gram-positive bacteria were identified in the
Set. No. 1, while only 7 in the Set No. 2. Contrastingly, Gram-negative
bacteria were much more abundant in the Set No. 2 (12 strains) than in
the Set No. 1 (2 strains). Bacillus sp. was the most common
Gram-positive strain (9 isolations from the first set, 2 isolations from
the second set). Pantoea agglomerans was the most common
Gram-negative strain (2 isolations from the first set, 7 isolations from
the second set). The first experience with the microbial profile of the
research station showed that we were able to detect mainly bacteria
commonly present in the outer environment that could survive under
extreme conditions. We did not isolate any microbes related to human
colonisation except of enterococci and Escherichia vulneris. For
further investigation of the station environment, it will be necessary
to choose alternative way of collection and storage of samples to ensure
survival of all present bacteria. |
Keywords: |
bacteria,
Bacillus sp., Pantoea agglomerans, James Ross
Island
|
References: |
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Notes: |