Title: | |
Authors Name: |
Sheetal Sharma, Rashmi Ranjan Sutar, Aseema Parida, Felix Bast |
Journal: | Czech Polar Reports |
Issue: | 11 |
Volume: | 2 |
Page Range: | 194-202 |
No. of Pages: | 9 |
Year: | 2021 |
DOI: |
10.5817/CPR2021-2-13 |
Publishers: | muniPress Masaryk University Brno |
ISSN: | 1805-0689 (Print), 1805-0697 (On-line) |
Language: | English |
Subject: | |
Abstract: |
Antarctica is the coldest and driest continent globally and has always been an exciting habitat to study extremophiles. The study reveals a monostromatic nitrophilic green alga Prasiola crispa (Trebouxiophyceae) growing on Adelie penguin guano at a penguin rockery, Larsemann Hills, Eastern Antarctica. This study is the first report of the barcode of this algal genus from Eastern Antarctica in general and the Larsemann Hills in particular. There are 35 species currently accepted in this genus, while four were reported from Antarctica. The present study relied on morphological diagnoses as well as the phylogenetic inference based on nuclear-encoded ITS gene and plastid-encoded tufA gene for species identification. The study generated phylogenetic reconstruction at the two selected loci for the first time for this species from Antarctica.
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Keywords: |
molecular phylogeny,
taxonomy, Polar region, tufA, ITS, green algae |
References: |
Alekseev, I., Zverev, A. and Abakumov, E. (2020): Microbial communities in permafrost soils of Larsemann Hills, Eastern Antarctica: Environmental controls and effect of human impact. Microorganisms, 8: 1202. doi: 10.3390/microorganisms8081202 Antonello, A. (2019): The greening of Antarctica: Assembling an international environment. Oxford University Press, USA. Print ISBN-13: 9780190907174. doi: 10.1093/oso/978019090 7174.001.0001 Bajerski, F., Wagner, D. (2013): Bacterial succession in Antarctic soils of two glacier forefields on Larsemann Hills, East Antarctica. FEMS Microbiology Ecology, 85(1): 128-142. doi: 10.1111/1574-6941.12105 Baldauf, S. L., Palmer, J. D. (1990): Evolutionary transfer of the chloroplast tufA gene to the nucleus. Nature, 344(6263): 262. doi: 10.1038/344262a0 Carvalho, E. L., Maciel, L. F., Macedo, P. E., Dezordi, F. Z., Abreu, M. E., Victoria, F. D. C., Pereira, A. B., Boldo, J. T., Wallau, G. D. L. and Pinto, P.M. (2018): De novo assembly and annotation of the Antarctic alga Prasiola crispa transcriptome. Frontiers in Molecular Biosciences, 4: 89. doi: 10.3389/fmolb.2017.00089 Choi, K. R., Roh, K., Kim, J. and Sim, W. (2000): Genomic cloning and characterization of mitochondrial elongation factor Tu (EF-Tu) gene (tufM) from maize (Zea mays L.). Gene, 257(2): 233-42. doi: 10.1016/s0378-1119(00)00404-2 Chown, S. L., Convey, P. (2007): Spatial and temporal variability across life's hierarchies in the terrestrial Antarctic. Philosophical Transactions of the Royal Society B: Biological Sciences, 362(1488): 2307-2331. doi: 10.1098/rstb. 2006.1949 Das, S. K., Singh, D. (2021): Epiphytic algae on the bryophytes of Larsemann Hills, East Antarctica. National Academy Science Letters, 44(2): 161-165. doi: 10.1007/s40009-020-00947-7 Dubrasquet, H., Garrido, I., Bruning, P., Reyes, J. and Guillemin, M. L. (2021): Building-up knowledge on green marine macroalgae diversity in the Western Antarctic Peninsula: Data from two molecular markers reveals numerous species with amphipolar distribution. Cryptogamie Algologie, 42(2): 21-37. doi: 10.5252/cryptogamie-algologie2021v42a2 Fernández-Marín, B., López-Pozo, M., Perera-Castro, A. V., Arzac, M. I., Sáenz-Ceniceros, A., Colesie, C., De Los Ríos, A., Sancho, L.G., Pintado, A., Laza, J. M. and Pérez-Ortega, S. (2019): Symbiosis at its limits: ecophysiological consequences of lichenization in the genus Prasiola in Antarctica. Annals of Botany, 124(7): 1211-1226. doi: 10.1093/aob/mcz149 Garrido-Benavent, I., de los Ríos, A., Fernández-Mendoza, F. and Pérez-Ortega, S. (2018): No need for stepping stones: Direct, joint dispersal of the lichen-forming fungus Mastodia tessellata (Ascomycota) and its photobiont explains their bipolar distribution. Journal of Biogeography, 45(1): 213-224. doi: 10.1111/jbi.13105 Garrido-Benavent, I., Pérez-Ortega, S. and de los Ríos, A. (2017): From Alaska to Antarctica: Species boundaries and genetic diversity of Prasiola (Trebouxiophyceae), a foliose chlorophyte associated with the bipolar lichen- forming fungus Mastodiatess ellata. Molecular Phylogenetics and Evolution, 107: 117-131. doi: 10.1016/j.ympev.2016.10.013 Gray, A., Krolikowski, M., Fretwell, P., Convey, P., Peck, L., Mendelova, M., Smith, A. G. and Davey, M. (2021): Remote sensing phenology of Antarctic green and red snow algae using WorldView satellites. Frontiers in Plant Science, 12: 877. doi: 10.3389/fpls.2021.671981 Gupta, P. (2015): Biodiversity of Larsemann Hills, Antarctica, Climate Change, 1(3): 174-183. Jackson, A., Seppelt, R. (2006): The accumulation of proline in Prasiola crispa during winter in Antarctica. Physiologia Plantarum, 94: 25-30. doi: 10.1111/j.1399-3054.1995.tb00 779.x Jackson, A. E., Seppelt, R. D. (1997): Physiological adaptations to freezing and UV radiation exposure in Prasiola crispa, an Antarctic terrestrial alga. In: B. Battaglia, J. Valencia, and D. W. H. Walton (eds.): Antarctic Communities: Species, Structure, and Survival, (Cambridge: University Press), pp. 226–233. Jacob, A., Kirst, G. O., Wiencke, C. and Lehmann, H. (1991): Physiological responses of the Antarctic green alga Prasiola crispa ssp. antarctica to salinity stress. Journal of Plant Physiology, 139(1): 57-62. Jacob, A., Wiencke, C., Lehmann, H. and Kirst, G. O. (1992): Physiology and ultrastructure of desiccation in the green alga Prasiola crispa from Antarctica. Botanica Marina, 35: 297-303. doi: 10.1515/botm.1992.35.4.297 Klaus, D. (2021): The Greening of Antarctica: Assembling an international environment, by Alessandro Antonello, The English Historical Review, 136(579): 461-463. doi: 10.1093/oso/ 9780190907174.001.0001 Moniz, M. B., Guiry, M. D. and Rindi, F. (2014): Tuf A phylogeny and species boundaries in the green algal order Prasiolales (Trebouxiophyceae, Chlorophyta). Phycologia, 53(4): 396-406. doi: 10.2216/13-233.1 Moniz, M. B., Rindi, F., Novis, P. M., Broady, P. A. and Guiry, M. D. (2012): Molecular phylogeny of Antarctica Prasiola (Prasiolales, Trebouxiophyceae) Reveals Extensive Cryptic Diversity. Journal of Phycology, 48(4): 940-955. doi: 10.1111/j.1529-8817.2012.01172.x Naw, M. W. D., Hara, Y. (2002): Morphology and molecular phylogeny of Prasiola sp. (Prasiolales, Chlorophyta) from Myanmar. Phycological Research, 50(3): 175-182. doi: 10.1046/ j.1440-1835.2002.00271.x Peter, C. (2010): Terrestrial biodiversity in Antarctica-Recent advances and future challenges. Polar Science, 4(2): 135-147. doi: 10.1016/j.polar.2010.03.003 Rindi, F., Guiry, M. D., Barbiero, R. P. and Cinelli, F. (1999): The marine and terrestrial Prasiolales (Chlorophyta) of Galway City, Ireland: a morphological and ecological study. Journal of Phycology, 35(3): 469-482. doi: 10.1046/ j.1529-8817.1999.3530469.x Rindi, F., McIvor, L. and Guiry, M. D. (2004): The Prasiolales (Chlorophyta) of Atlantic Europe: An assessment based on morphological, molecular, and ecological data, including the characterization of Rosenvingiella radicans (KÜTZING) comb. nov. Journal of Phycology, 40(5): 977-997. doi: 10.1111/j.1529-8817.2004.04012.x Shibistova, O., Boy, J., Godoy, R., Boy, D., McCulloch, R., Mikutta, R. and Guggenberger, G. (2017): Greening boosts soil formation and soil organic matter accumulation in Maritime Antarctica. https://eprints.dbges.de/id/eprint/1775 Wiencke, C., Amsler, C. D. (2012): Seaweeds and Their Communities in Polar Regions. In: C. Wiencke, K. Bischof (eds): Seaweed Biology. Ecological Studies (Analysis and Synthesis), 219: 265-291. Springer, Berlin, Heidelberg. doi: 10.1007/978-3-642-28451-9_13 Wiencke, C., Amsler, C. D. and Clayton, M. N. (2014): Macroalgae. In: C. De Broyer, P. Koubbi, H. Griffiths, B. Raymond, C. Udekem d*Acoz (eds.): Biogeographic Atlas of the Southern Ocean., Biogeographic Atlas of the Southern Ocean., Cambridge, Scientific Committee on Antarctic Research, 8 p., ISBN: 978-0-948277-28-3
Web sources / Other sources [1]
ImageJ software
[2]
Geneious
software v 8.0 (Created by Biomatters) |
Notes: |