Tryptone 10 g
Beef extract 5 g
Yeast extract 5 g
Glucose 7 g
Fructose 7 g
Maltose 7 g
Sodium gluconate 2 g
Sodium acetate.3H2O 5 g
Diamonium citrate 2 g
K2HPO4.3H2O 2.6 g
MgSO4.7H2O 0.1 g
MnSO4.4H2O 0.05 g
L-Cysteine.HCl 0.5 g
Tween 80 1 ml
Distilled water 1000 ml
pH 6.3
Sterilize at 115°C for 20 min.