Medium 115: TGA MEDIUM

    Trypton                       10 g
    NaCl                           5 g
    HEPES-buffer 
       (Fluka No.54461)         11.9 g
    Glucose                        2 g
    Agar                          30 g
    Distilled water             1000 ml
    pH 7.0

Sterilize at 115°C for 20 min., cool to 50°C and 
add sterile 60 mg ampicillin/1L.