Medium 131: CDM MEDIUM
I. Solution
MgSO4.7H20 2 g
NH4Cl 1 g
Na2SO4 0.99 g
K2HPO4 0.01 g
CaCl2.2H20 0.067 g
Na lactate 0.05 g
Deionized water 100 ml
Sterilization: at 121°C for 15 min.
II. Solution:
FeSO4.7H20 0.13 g
Deionized water 100 ml
Sterilization: Millipore filter
III. Solution:
NaHCO3 7.98 g
Deionized water 100 ml
Sterilization: Millipore filter
Add 20 g the agar and 900 ml distilled water to
solution I and sterilizite at 120 C for 15 min.,
final pH 7.2. Add aseptically 2.5 ml solution II.
and 10 ml solution III. to the warm solution I.
with the agar and distribute the medium into
sterile tubes or Petri dishes.