Medium 133: HALOALKALIPHILE MEDIUM II

 I. Yeast extract                  5 g
    Peptone                        5 g
    Glucose                       10 g
    KH2PO4                         1 g
    MgSO4.7H2O                   0.2 g
    NaCl                          80 g
    Distilled water              800 ml
    Agar                          20 g

II. Na2CO3                        20 g
    Distilled water              200 ml

    Sterilize I. and II. at 115 C for 15 min.,
    cool to 60 C and mix together aseptically.
    Do not adjust pH.