Medium 143: MARINE MEDIUM

    Bacto peptone              5 g
    Bacto yeast extract        1 g
    Fe(III) citrate          0.1 g
    NaCl                   19.45 g
    MgCl2 (dried)            5.9 g
    Na2SO4                  3.24 g
    CaCl2                    1.8 g
    KCl                     0.55 g
    Na2CO3                  0.16 g
    KBr                     0.08 g
    H3BO3                     22 mg
    (NH4)NO3                 1.6 mg
    Na2HPO4                    8 mg
    Distilled water         1000 ml

    Adjust pH to 7.6 +- 0.2
    Sterilize at 115C for 20 min.