Bacto peptone 5 g
Bacto yeast extract 1 g
Fe(III) citrate 0.1 g
NaCl 19.45 g
MgCl2 (dried) 5.9 g
Na2SO4 3.24 g
CaCl2 1.8 g
KCl 0.55 g
Na2CO3 0.16 g
KBr 0.08 g
H3BO3 22 mg
(NH4)NO3 1.6 mg
Na2HPO4 8 mg
Distilled water 1000 ml
Adjust pH to 7.6 +- 0.2
Sterilize at 115°C for 20 min.