Medium 145: PROSTHECOMICROBIUM MEDIUM

    NH4H2PO4              0.03 g
    Na2HPO4              0.071 g
    KH2PO4               0.036 g
    MgSO4                 0.03 g
    Solution of metals     0.1 ml
    Yeast extract         0.03 g
    Glucose               0.03 g
    Distilled water        100 ml

    Adjust pH to 7. 
    Sterilize at 118°C for 15 min.   

    Solution of metals:
    EDTA                  0.25 g
    ZnSO4.7H2O             1.1 g
    FeSO4.7H2O             0.5 g
    MnSO4.H2O            0.154 g
    CuSO4.5H2O            0.04 g
    Co(NO3)2.6H2O        0.025 g
    Na2B4O7.10H2O        0.018 g
    Distilled water        100 ml