Medium 147: LEUCOTHRIX MEDIUM

    Trypton                   10 g
    Synthetic sea water     1000 ml

Synthetic sea water:
    NaCl                      24 g
    MgCl.6H2O                 11 g
    Na2SO4                     4 g
    CaCl2.6H2O                 2 g
    KCl                      0.7 g
    KBr                      0.1 g
    H3BO3                   0.03 g
    NaSiO3.9H2O                5 mg
    SrCl.6H2O               0.04 g
    NaF                        3 g
    NH4NO3                     2 g
    Fe3PO4.4H2O                1 g
    Distilled water         1000 ml

    Adjust pH to 7.8.  
    Sterilized at 121C for 15 min.