NaCl 39 g
MgCl2.6H2O 6.5 g
MgSO4.7H2O 10.1 g
KCl 1 g
NaBr 0.1 g
NaHCO3 0.03 g
CaCl2.2H2O 0.16 g
Yeast extract 5 g
Distilled water 1000 ml
Adjust pH to 7.2 - 7.4.
Sterilized at 121°C for 15 min.