R2A agar (Difco) 18.2 g SW30 500 ml Distilled water 500 ml agar 2 g Adjust pH to 7.5 Sea Water Solution 30% (SW30) NaCl 234 g MgCl.6H2O 39 g MgSO4.7H2O 61 g CaCl2 1 g KCl 6 g NaHCO3 0.2 g NaBr 0.7 g Distilled water 1000 ml