Medium 18: AZOTOBACTER MEDIUM

    Mannitol                      10 g 
    K2HPO4                       0.2 g 
    MgSO4.7H2O                   0.2 g 
    CaSO4                        0.1 g 
    NaCl                         0.2 g 
    CaCO3                          5 g 
    Agar                          20 g 
    Tap water                   1000 ml