Medium 24: THIOBACILLUS MEDIUM

    Na2HPO4.7H2O                 7.9 g 
    KH2PO4                       1.5 g 
    NH4Cl                        0.3 g 
    MgSO4.7H2O                   0.1 g 
    Trace element solution*        5 ml 
    Phenol red                     2 mg 
    Na2S2O3.5H2O                  10 g 
    Agar                          20 g 
    Distilled water             1000 ml 
 
Sterilize separately the trace elements and 
MgSO4.7H2O. Adjust pH to 8.5 with 10% sterile 
NaHCO3. 
 
   *Trace element solution:
    Ethylenediaminetetraacetic
    acid                          50 g
    ZnSO4.7H2O                    22 g
    CaCl2                       5.54 g
    MnCl2.4H2O                  5.06 g
    FeSO4.7H2O                  4.99 g
    (NH4)6Mo7O24.4H2O            1.1 g
    CuSO4.5H2O                  1.57 g
    CoCl2.6H2O                  1.61 g
    Distilled water             1000 ml
 
Adjust pH to 6.0 with KOH.