Yeast extract 2 g Proteose peptone No.3 2 g Casitone 2 g Na2HPO4.12H2O 2.5 g KH2PO4 1 g Sodium citrate 1.5 g MgSO4.7H2O 0.6 g Tween 80 0.5 g Glycerol 50 ml Agar 20 g Distilled water 1000 ml pH 7.0