Medium 5: TOMATO JUICE AGAR

    Peptone                         10 g 
    Casein peptone, tryptic digest  10 g 
    Tomato juice*              	   400 ml
    Agar                            20 g 
    Distilled water                600 ml 
 
Dissolve the solids in water by heating. Add 
tomato juice, mix, and sterilize at 121C for 20 
min. The final pH value of the medium should be 
6.0 - 6.2.
 
     *Tomato juice:
     Tomatoes                       250 g
     Distilled water                500 ml
 
Cut up tomatoes and steam in water for an hour or 
until they are pulped. Clarify through gauze and 
filter through paper.