Medium 50: VL MEDIUM WITH BLOOD

    Yeast extract                  5 g 
    Beef extract                   2 g 
    Tryptone                      10 g 
    Glucose                        2 g 
    NaCl                           5 g 
    L-Cysteine.HCl               0.3 g 
    Agar                          20 g 
    Distilled water             1000 ml 
 
Adjust to pH 7.4. Sterilize at 115C for 20 min., 
cool to 50C and add 100 ml sterile sheep or horse
 blood.