Medium 6 - LACTOBACILLUS MEDIUM (MRS)

    Yeast extract                  5 g 
    Beef extract                  10 g 
    Peptone                       10 g 
    Glucose                       20 g 
    Tween 80                       5 ml 
    K2HPO4                         2 g 
    Sodium acetate                 5 g 
    Diamonium citrate              2 g 
    MgSO4.7H2O                   0.2 g 
    MnSO4.4H2O                  0.05 g 
    Distilled water             1000 ml
 
Dissolve the ingredients in water and adjust pH to
 6.2 - 6.6. Distribute into tubes in high 
layer.