Peptone 1 g
Yeast extract 1 g
Nitrilotriacetic acid 0.1 g
CaSO4.2H2O 0.06 g
MgSO4.7H2O 0.1 g
NaCl 0.008 g
KNO3 0.103 g
NaNO3 0.689 g
Na2HPO4 0.111 g
FeCl3 (0.03 g/l) 1 ml
Trace element solution* 1 ml
Agar 30 g
(or 1.1% Gelrite + 0.1% CaCl2.2H2O)
Distilled water 1000 ml
pH 8.2
*Trace element solution:
MnSO4.2H2O 0.22 g
ZnSO4.7H2O 0.05 g
H3BO3 0.05 g
CuSO4 1.6 mg
CoCl2.6H2O 4.6 mg
Na2MoO4 2.5 mg
Distilled water 100 ml