Medium 9: HALOBACTERIUM - HALOCOCCUS MEDIUM
I. Skim milk (Difco) 50 g
Distilled water 500 ml
II. Salt solution:
MgSO4.7H2O 10 g
KNO3 2 g
NaCl 200 g
Ferric citrate traces
Distilled water 100 ml
III. Neopeptone 5 g
Glycerol 10 ml
Agar 25 g
Distilled water 400 ml
Steam separately the three solutions. Mix together
II. and III. and adjust pH to 8.4. Sterilize the
agar - salt mixture at 121°C for 20 min. Add the
hot mixture to the warm skim milk aseptically.
Distribute the medium into sterile tubes or Petri
dishes.