Medium 9: HALOBACTERIUM - HALOCOCCUS MEDIUM

    I. Skim milk (Difco)          50 g 
    Distilled water              500 ml 

    II. Salt solution: 
    MgSO4.7H2O                    10 g 
    KNO3                           2 g 
    NaCl                         200 g 
    Ferric citrate              traces 
    Distilled water              100 ml
 
    III. Neopeptone                5 g 
    Glycerol                      10 ml 
    Agar                          25 g 
    Distilled water              400 ml

Steam separately the three solutions. Mix together
 II. and III. and adjust pH to 8.4. Sterilize the 
agar - salt mixture at 121°C for 20 min. Add the 
hot mixture to the warm skim milk aseptically. 
Distribute the medium into sterile tubes or Petri 
dishes.