Nutrient broth 13 g
Yeast extract powder 3 g
Peptone 5 g
Distilled water 1000 ml
Adjust pH to 7.4 +/- 0.2.
Sterilized at 121°C for 15 min.
Add sterile solution of CaCl2 (stock solution
0.02M) to final concentration 0.002M.