Ammonium tartrate 5 g NH4NO3 1 g KH2PO4 1 g MgSO4 0.5 g NaCl 0.1 g CaCl2 0.1 g Sucrose 15 g Biotin 5 x 10-6 g Bo 0.01 mg Cu 0.1 mg Fe 0.2 mg Mn 0.02 mg Mo 0.02 mg Zn 2 mg Distilled water 1000 ml Autoclave at 121°C for 20 min.