NaNo3 2 g K2HPO4 1 g KCl 0.5 g MgSO4.7H2O 0.5 g FeSO4 0.01 g Sucrose 200 g Agar 20 g Distilled water 1000 ml If glass distilled water is used, add 1 ml of each solution prepared as follows: ZnSO4 0.01 g CuSO4 0.005 g Distilled water 1000 ml Autoclave at 121 °C for 20 min.