Milk powder risk assessment with staphylococcus aureus toxigenic strains

Publikace nespadá pod Přírodovědeckou fakultu, ale pod Lékařskou fakultu. Oficiální stránka publikace je na webu muni.cz.

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BOGDANOVIČOVÁ Kateřina NECIDOVÁ Lenka HARUŠTIAKOVÁ Danka JANŠTOVÁ Bohumíra

Druh Článek ve sborníku
Konference 29th International Conference of the European-Federation-of-Food-Science-and-Technology (EFFoST)
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
Doi http://dx.doi.org/10.1016/j.foodcont.2016.07.007
Obor Ostatní lékařské obory
Klíčová slova Staphylococcal enterotoxins; Powdered milk; ELFA
Popis Staphylococcus aureus is the causative agent of staphylococcal enterotoxicosis foodborne illnesS. Milk powder and whey powder are at risk of contamination by coagulase-positive staphylococci, which reflects the requirement for microbiological examination of foods listed in Regulation (EC) 2073/2005, as amended. Microbiological criteria for coagulase-positive staphylococci are up to 10(1)-10(2) cfu g(-1). This study evaluates the possibility of survival and growth of S. aureus in milk powder after its reconstitution. Powdered milk was inoculated with 10(2) and 10(5) cfu g(-1) of toxigenic strains of S. aureus and then stored as reconstituted milk for 48 h at 4,15, and 25 degrees C. Staphylococcal growth and production of staphylococcal enterotoxins A, B, and C (SEA, SEB, and SEC) was regularly detected during the 48 h storage period. With inoculation of S. aureus high counts at 25 degrees C, the production of staphylococcal enterotoxins (SEs) was detected as early as after 7 (SEB) or 8 (SEA) hours of storage; at 15 degrees C as early as after 48 h (SEA, SEB). With inoculation of low counts of S. aureus (complying with legislative requirements) only at 25 degrees C, SEs production was detected after 24 (SEA, SEB) or 48 (SEA, SEB, SEC) hours. Model experiments evaluated SEs consumer risk resulting from extended storage of reconstituted milk at improper temperatures. (C) 2016 Elsevier Ltd. All rights reserved.

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