De-novo development of microsatellite markers for Dactylogyrus vistulae

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GETTOVÁ Lenka VETEŠNÍKOVÁ ŠIMKOVÁ Andrea

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Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
Popis In our study, we aimed to characterize de-novo microsatellite markers for Dactylogyrus parasites. Dactylogyrus vistulae was selected as a model organism owing to its relatively large body size in comparison to the other members of this genus. Approximately 300 specimens of D. vistulae were collected from European chub Squalius cephalus of the Dyje River. Genomic DNA was isolated and subsequently prepared genomic libraries were subjected to Illumina MiSeq (provided by CEITEC, MU, Brno). QDD program (Meglécz et al. 2010) was used for selecting microsatellite markers and primer design. Following criteria used in Gettová et al. (2013) 70 sequences with target microsatellites were selected. Monogenean parasites, especially dactylogyrids, display a high level of host specificity. As a result genetic structure of monogenean parasites might be host-dependent. Microsatellite markers developed for Dactylogyrus parasites might, therefore, enable us to reconstruct the evolution history of their fish hosts. In addition, in the case of rare generalist Dactylogyrus parasites (such is D. vistulae), we expect that the microsatellites will represent a useful tool to analyse the structure of parasite populations parasitizing different host species and/or geographically isolated parasite populations across Europe.
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