Changes in the structure of interphase nucleai of human leukemic cell lines after treatment with differentiating agents and gama irradiation

Information

This project doesn't include Faculty of Science. It includes Faculty of Informatics. Official project website can be found on muni.cz.

Investor logo
Project Identification
GA202/98/P253
Project Period
1/1999 - 1/2001
Investor / Pogramme / Project type
Czech Science Foundation
MU Faculty or unit
Faculty of Informatics
Keywords
later
Cooperating Organization
Institute of Biophysics

The main objective of the proposed grant is the research of the changes of the nuclear chromatin structure in the human leukemic myeloid cell lines after the treatment with differentiating agents and after gamma-irradiation. Changes of abl, bcr and c-myc gene topology in cell nuclei will be detected. Translocation t(9,22);(q34,q11) which leads to chimeric bcr-abl gene (Philadelphia chromosome)that plays an important role in the pathogenesis of chronic myelogenous leukemia (CML) will be studied. Fluoresc ence in situ hybridisation (FISH) combined with the image analysis will be used. Changes in the structure of interphase nuclei will be related to the cell cycle profile and to the stages of cell maturation. In the frame of this project, the reverse trans cription polymerase chain reaction (RT-PCR) for c-myc and abl-bcr gene expression will be applied. C-myc protooncogene plays an essential role in the regulation of G1 phase of the cell cycle and is important in the initiation of myeloid differentiation a 

Publications

Total number of publications: 4


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