Project information
Polymikrobiální biofilm jako komplexní mikrobiom v rámci léčby nozokomiálních infekcí
(Biofilm)
- Project Identification
- 16-31593A (kod CEP: NV16-31593A)
- Project Period
- 4/2016 - 3/2020
- Investor / Pogramme / Project type
-
Ministry of Health of the CR
- Ministry of Health Research Programme 2015 - 2022
- MU Faculty or unit
- Faculty of Medicine
- Cooperating Organization
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Centre for Cardiovascular and Transtplantation Surgary
- Responsible person prof. MUDr. Tomáš Freiberger, Ph.D.
- Responsible person prof. MUDr. Filip Růžička, Ph.D.
Catheterization of patient is common during many interventions. This is one of reasons, why over 40% of nosocomial infections are infections of urinary tract, esp. of catheterized patients. Poly-microbial wound infection are known to be only partially cultivable, part of microbes can be proved only by molecular techniques. The microbial diversity of biofilm communities of central venous catheters was not satisfactory examined. Suitable method for poly-microbial diagnostic may be ARISA, T-RFLP and/or DGGE; with NGS. In most studies the genetic examination proved also non-cultivable bacteria and even bacteria unknown with those infections. The aim of this project is to study of species composition of biofilm communities by cultivation and molecular methods and assessment of the differences in poly-microbial communities in relation to therapy failure, length of insertion of catheter, its type and other factors including predominating microbial species.
Publications
Total number of publications: 17
2021
2020
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Prevalence of bacteriocins and their co-association with virulence factors within Pseudomonas aeruginosa catheter isolates
International Journal of Medical Microbiology, year: 2020, volume: 310, edition: 8, DOI
2019
-
Actinotignum schaalii as a part of urinary catheter associated microbiota
Year: 2019, type: Conference abstract
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Capability of Fluorescent Capillary Electrophoresis To Distinguish Species of the Candida parapsilosis Complex
Journal of Clinical Microbiology, year: 2019, volume: 57, edition: 5, DOI
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Fluorescent Capillary Electrophoresis Applied to Distinguish Species of Candida parapsilosis Complex
Year: 2019, type: Conference abstract
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Fluorescent Capillary Electrophoresis Is Superior to Culture in Detecting Candida Species from Samples of Urinary Catheters and Ureteral Stents with Mono- or Polyfungal Biofilm Growth
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Identification of ability to form biofilm in Candida parapsilosis and Staphylococcus epidermidis by Raman spectroscopy
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Molecular Techniques Complement Culture-Based Assessment of Bacteria Composition in Mixed Biofilms of Urinary Tract Catheter-Related Samples
2018
2017
-
Biofilm formation, antibiotic susceptibility and RAPD genotypes in Pseudomonas aeruginosa clinical strains isolated from single centre intensive care unit patients
Folia microbiologica, year: 2017, volume: 62, edition: 6, DOI