Van Gogh-like (Vangl) and Prickle (Pk) are core components of the non-canonical Wnt planar cell polarity pathway that controls epithelial polarity and cell migration. Studies in vertebrate model systems have suggested that Vangl and Pk may also inhibit signaling through the canonical Wnt/β-catenin pathway, but the functional significance of this potential cross-talk is unclear. In the nematode C. elegans, the Q neuroblasts and their descendants migrate in opposite directions along the anteroposterior body axis. The direction of these migrations is specified by Wnt signaling, with activation of canonical Wnt signaling driving posterior migration, and non-canonical Wnt signaling anterior migration. Here, we show that the Vangl ortholog VANG-1 influences the Wnt signaling response of the Q neuroblasts by negatively regulating canonical Wnt signaling. This inhibitory activity depends on a carboxy-terminal PDZ binding motif in VANG-1 and the Dishevelled ortholog MIG-5, but is independent of the Pk ortholog PRKL-1. Moreover, using Vangl1 and Vangl2 double mutant cells, we show that a similar mechanism acts in mammalian cells. We conclude that cross-talk between VANG-1/Vangl and the canonical Wnt pathway is an evolutionarily conserved mechanism that ensures robust specification of Wnt signaling responses. Více informací
Similar Posts
Bioactive Excreted/Secreted Products of Entomopathogenic Nematode Heterorhabditis bacteriophora Inhibit the Phenoloxidase Activity during the Infection
Insects 2020, 11(6), 353; https://doi.org/10.3390/insects11060353 ABSTRACT: Entomopathogenic nematodes (EPNs) are efficient insect parasites, that are... Read More
Coordinated labio-lingual asymmetries in dental and bone development create a symmetrical acrodont dentition
Abstract Organs throughout the body develop both asymmetrically and symmetrically. Here, we assess how symmetrical... Read More
Role of casein kinase 1 in the amoeboid migration of B-cell leukemic and lymphoma cells: A quantitative live imaging in the confined environment
Abstract: The migratory properties of leukemic cells are commonly associated with their pathological potential and... Read More
An RNA aptamer restores defective bone growth in FGFR3-related skeletal dysplasia in mice
Abstract: Achondroplasia is the most prevalent genetic form of dwarfism in humans and is caused... Read More
Carboxy-terminal polyglutamylation regulates signalling and phase separation of the Dishevelled protein
Abstract Polyglutamylation is a reversible posttranslational modification that is catalyzed by enzymes of the tubulin... Read More
Changes in haemolymph parameters and insect ability to respond to immune challenge during overwintering
Abstract: Overwintering is a challenging period in the life of temperate insects. A limited energy... Read More