Nanotechnological and bioanalytical detection of the DNA damage

Project Identification
2B06056
Project Period
7/2006 - 6/2010
Investor / Pogramme / Project type
Ministry of Education, Youth and Sports of the CR
MU Faculty or unit
Faculty of Science
Keywords
polyaromatické uhlovodíky, poškození DNA, adukty, zhoubné bujení, protilátka, biokonjugáty, nanočástice, mikroskopie atomové síly, afinitní biosensory, povrchová resonannce plasmonů, ELISA

The aim of the project is development of novel methods for detecton of DNA damage resulting from exposure to polyaromatic hydrocarbons (PAH). Initially, PAH-DNA aducts will be prepared under enzymatic catalysis of cytochrome P450 and using chemical synthesis. The aducts will be used to rise specific antibodies (monoclonals as well as polyclonals) which will further serve for development of immunochemical methods including ELISA (standard, nanoparticle- and enzyme recyclation-amplified variants, opticaland electrochemical detection) and immunosensors (surface plasmon resonance system Biacore, piezoelectric resonators). Biosensor-based technologies will also serve for detailed characterization of the newly prepared bioreagents. The localization of DNA damage will be studied using atomic force microscopy, nanoparticle-labeled antibodies will serve for visualization of the point of damage. Finally, the newly developed detection methods will be tested on real samples and validated with alternative approaches.

Publications

Total number of publications: 17


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