Identification and characterization of proteins involved in metabolism of G-quadruplexes and R-loops and molecular mechanisms of their relationship with replication

Information

This project doesn't include Faculty of Science. It includes Faculty of Medicine. Official project website can be found on muni.cz.

Project Identification
21-22593X
Project Period
1/2021 - 12/2025
Investor / Pogramme / Project type
Czech Science Foundation
MU Faculty or unit
Faculty of Medicine
Cooperating Organization
Institute of Molecular Genetics of the ASCR, v. v. i.

DNA replication is an essential and one of the most complex processes in the cell. Not only exogenous DNA damage but also intrinsic DNA structures including G-quadruplexes (G4) and R-loops, their stabilization or unscheduled formation represent major replication obstacles with possible detrimental effects on genome integrity. Not surprisingly, those processes are pharmacologically targeted in anticancer therapy, despite the fact that only little is known about the underlying molecular mechanisms. It becomes apparent that maintenance of processive DNA replication requires sophisticated protein networks beyond the core replisome. Whether there is a direct crosstalk between G4 and R-loops, what proteins are involved in their homeostasis and what are the factors diversifying between their beneficial and pathological roles, is not well understood. The goals of our research are to identify proteins associated with G4 and R-loop structures and understand their roles in G4/R-loop formation and resolution as well as relationship to replication fork progression and associated repair.

Publications

Total number of publications: 1


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